Tag

Part:BBa_K1696013

Designed by: Yue Liu   Group: iGEM15_TJU   (2015-09-14)

Mf-ssrA Tag1


Mf-ssrA tag1 is an identified pdt (protein degradation tag) variant that alters mf-Lon-dependent degradation but not recognition by endogenous E.coli proteases. In our experiment, we use tag1 as the parental tag and targeted pdt residues 13-15 further for mutagenesis because this region is essential for Lon-mediated degradation in Mycoplasma pneumonia and does not show homology to known ClpA, ClpX or SspB binding sites.[1]


References

Cameron D E, Collins J J. Tunable protein degradation in bacteria[J]. Nature biotechnology, 2014, 32(12): 1276-1281.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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